histone 3 abcam ab18521 1 5000  (Danaher Inc)

Journal: Scientific Reports

Article Title: Premature aging effects on COVID-19 pathogenesis: new insights from mouse models

doi: 10.1038/s41598-024-70612-2

Figure Lengend Snippet: Characterization of HGPS/hACE2 mice. ( A ) Schematic diagram of HGPS/hACE2 mouse generation strategy using mES editing combined with tetraploid complementation techniques. ( B ) Western blot analysis of lung tissues from hACE2 and HGPS/hACE2 mice, showing the presence of Lamin A/C in wild type lung and progerin in HGPS lungs. Histone 3 was used as a loading control. ( C ) Representative photographs of 2-month-old unedited, heterozygous, and homozygous Lmna c.1827C < T mutant hACE2 mice. ( D ) Survival curve plot showing the life span of wild type, heterozygous and homozygous Lmna mutant mice. D = days ( E ) Boxplots illustrating the body weight distribution among young, aged, and HGPS Balb/c mice. ( F ) Volcano plot showing transcriptome changes in the lungs between hACE2 and HGPS/hACE2 mice. Significantly differentially expressed genes are highlighted in green (upregulated in HGPS) and red (upregulated in wild type). ( G ) Dropblot of GO analysis results derived from differentially expressed gene sets in the lungs of 2-month-old hACE2 and HGPS/hACE2 mice.

Article Snippet: The following primary antibodies were used: anti-LAMINA/C (CST, 4777S, 1:1000), anti-progerin (abcam, ab66587, 1:1000), Histone 3 (abcam, ab18521, 1:5000).

Techniques: Western Blot, Control, Mutagenesis, Derivative Assay